FTIR Analysis of Lipid Produced by Oleaginous Yeast Lipomyces starkeyi Using Agricultural Waste
Murugan S.
Department of Environmental Engineering,GCT, Coimbatore , IN
Sivasakthi S
Department of Environmental Engineering,GCT, Coimbatore , IN
Keywords: Agriculture waste, Hydrolysis, oleaginous yeast, biomass, Lipid.
Abstract
Biodiesel is obtained from a chemical reaction called transesterification (ester exchange). The reaction converts esters from long chain fatty acids into mono alkyl esters. Chemically, biodiesel commonly is a fatty acid methyl ester. Lipid is a class of organic compounds that are fatty acids or their derivatives and are insoluble in water but in organic solvents. Lipids include fatty acids, oils, waxes, sterols and triglycerides. Biodiesel is typically made by chemically reaction on lipids with an alcohol producing fatty acid esters. Lipids from agricultural waste is used as a resource for the preparation of biodiesel. The waste has a hard husk protecting the kernel inside. It contains 25% Cellulose, 30% lignin, 15% hemicellulose and 21% ash. Agricultural Waste was dried and ground in a mixer and further ground for 1hr in ball mill. Then they were dried and subjected to hydrolysis techniques such as acid and alkaline hydrolysis. After hydrolysis pH was adjusted as required for the species. After pH adjustment, media was autoclaved and inoculated with 6% of microorganism. In this study, hydrolyzed agricultural wastes were used for culturing oleaginous yeast Lipomyces starkeyi. UV visible spectroscopy is used to find the growth curve of microbes. Lipid extraction is done from the biomass obtained on acid hydrolysis and characterized by FTIR. It confirms the presence of lipid.
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